Multi-Model Protein Interaction Screening

1 Product Overview

Multi-Modal Protein Interaction Screening is an innovative research platform that integrates advanced tools and multi-level screening methods, aiming for efficient prediction and precise selection of protein interactions. This product employs cutting-edge technologies such as MegaDock, HDock, and AlphaFold, utilizing a multi-level screening strategy to systematically eliminate unsuitable protein interaction candidates from the comprehensive protein digital library.

In addition, the platform emphasizes two core factors: binding energy and structural prediction, enhancing the scientific rigor and comprehensiveness of the screening results. This systematic screening process provides new insights and methodologies for exploring the mechanisms of protein interactions, uncovering the biological basis of diseases, and advancing fundamental scientific research.

2 Product Screening Workflow

2.1 MegaDock Initial Docking Screening

As a preliminary screening tool, MegaDock enables the rapid analysis of large-scale protein interaction data. Through efficient docking simulations, it identifies protein interaction candidates with significant potential. The Top 200 candidates are selected for the next round of screening.

2.2 HDock Refined Docking Screening

HDock performs a more refined screening of the preliminary results, evaluating the stability and affinity of protein interactions based on absolute binding energy scores. This step further narrows the selection, focusing on high-performing candidates. The Top 10 candidates are selected for the next round of analysis.

2.3 AlphaFold One-to-One Precision Analysis

In the final refined analysis, AlphaFold plays a critical role by calculating pTM and ipTM scores. These metrics validate the reliability and biological significance of the screening results by predicting protein complex structures and modeling binding interfaces.

3 Deliverables